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ISSN: 2320 –7825 (Print); eISSN 2320 –7833 (Online)
Applied Biology and Biotechnology
Peer - Reviewed Journal
Volume No. 1(3) December,2013
Antibacterial activity of Helimeda macroloba extract against infectious pathogens
A.Pushparaj, D. Rajan, R.S.Raubbin, I. Vasudhevan, and P. Ambika
Abstract
The green seaweed Helimeda macroloba Decne,1841 was collected from Gulf of Mannar, Tuticorin coast. In this study, the selected seaweed were tested for probable antimicrobial activity through agar well diffusion method and the extracts were prepared by five different solvents viz., Acetone, Chloroform, Ethanol, Ethyl acetate and methanol; screened against six infectious pathogens (Gram +ve: Staphylococcus aureus, Bacillus subtilis, Lactobacillus acidophilus and Gram -ve: Pseudomonas aeruginosa, Escherichia coli and Proteus mirabilis). The experimental results shows that the highest antibacterial activity 16mm (100µl) was shown by Ethyl acetate extract against Proteus mirabilis and the lowest activity were seen in Chloroform extract 4mm (100µl) against Pseudomonas aeruginosa. The experiment concludes that Ethanol as a solvent only shows good activity against all the tested pathogens.