Botanical Report
International Journal of Plant Sciences
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  • ISSN:  2319-8230 (Print)
GTRP- BIOJOURNALS

Peer - Reviewed Journal

Micropropagation of rare orchid, Eulophia epidendraea (Retz.)Fischer

M. Maridass, G. Raju, R. Mahesh and K. Muthuchelian 

Ethnomedicinal plant knowledge of  Tribe Muthuvas of Mannavan Shola Forest of Southern Western Ghats, Kerala, India

Binu Thomas, A. Rajendran, U. M. Chandrashekara and R.Sivalingam

In vitro propagation of Commelina ensifolia R.Br.

R. Mahesh, K. Muthuchelian, M. Maridass and G. Raju

Chasmophytes: the Potential plants for Rock gardening, from Velliangiri Hills of Southern Western Ghats, Tamil Nadu, India

Binu Thomas, Rajendran, A. and V. Aravindhan

Volume No. 1(1)   September, 2012

 

 

Table and Contents

A simple and reliable procedure for in vitro clonal propagation of orchid Eulophia epidendraea was studied. The explants of pseudo bulbs of E. epidendraea were cultured on Murashige and Skoog medium (MS) and media supplemented with different plant growth regulators (BAP, NAA and IBA). In the present study, the highest shoot number formed on the MS medium supplemented with 0.5 mg l-1 BAP + 0.25 mg l-1 IBA (43.75 shoots / 20 explant) and multiple shoot induction of best auxin combinations of 0.5 IBA mg/L-1 + 0.1 NAA mg/L-1 were observed. The plantlets of E. epidendraea were acclimatized and transplanted to greenhouse and more than 89% survival rate was observed.

 

Key words: Eulophia epidendraea (Retz.) Fischer, Micropropagation

 

Abbreviation: BAP: 6-benzylaminopurine; IBA: indole-3-butyric acid; NAA - 1-naphthaleneacetic acid ; MS - Murashige and Skoog Medium

Abstract
Ethnomedicinal data of 38 plant species used to prepare medicinal formulations to cure various ailments were gathered from Muthuva tribes who reside in the shola regions of Mannavan Shola Forests of Kerala. The present study discussed the persistence of folk medicine practices in Mannavan shola forest are being influenced by culture and socio-economic aspects, providing a cheaper and accessible alternative to the high cost pharmaceutical remedies. Key words: Ethnomedicine, Muthuvas, Mannavan shola forest, Kerala

Abstract

Commelina ensifolia R.Br. is a rare edible herb belonging to family Commelinaceae. In the present study, in vitro regeneration of C. ensifolia. from nodal explants has been established. Nodal explants were cultured on MS medium supplemented with different combinations of BAP, NAA and IBA. The nodal explant of C. ensifolia is responsible for root induction, mean number of roots and root length and mean number of elongated shoots and shoot length. In the observed results, C. ensifolia 90% showed root regeneration responses in IBA 3.0 mg/L-1 and NAA 1.0 mg/L-1. A well developed micro shoots and rooting media for complete regeneration of Commelina ensifolia has been developed in the present study.

Abbreviations: Commelina ensifolia, micropropagation; BAP: 6-benzylaminopurine; NAA:1- naphthaleneacetic acid IBA: indole-3-butyric acid; MS: Murashige and Skoog Medium.

Abstract
The present paper highlights the 30 ornamental chasmophytic species belonging to 20 families were collected from the rock crevices of Velliangiri Hills of Southern Western Ghats of Tamil Nadu India. These potential ornamental chasmophytic plants posesess an attractive habit, foliage and flowers, it also possess certain biological peculiarities for adapting rock crevices. Keywords: Wild ornamental, Chasmophytes, Rockery, Velliangiri Hills, Southern Western Ghats,