International Journal of  

                       Applied Bioresearch (IJAB)

ISSN 2250 - 2033 (Print); e -ISSN: 2250 - 2041 (Online)

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INDIA
GTRP- BIOJOURNALS

The quarterly peer-reviewed journal

Clonal propagation of Tylophora indica - A medicinal plant

R. Mahesh, K.Muthuchelian, M. Maridass and G.Raju

Phylogenetic identification of antimicrobial active marine bacteria from sediments off the coast of South East India

R. Saravanakumar, J. Ronald, K. Maheswari and U. Ramesh

Nephroprotective activity of aqueous extract of Solanum nigrum in Amphotericin B induced Wister rats

A. Geo vigila and X. Baskaran

Effect of pre-storage treatment on seed germination of Strychnos potatorum L.f.

R. Sivalingam, D. Patric Raja, V. Irudayaraj and K.V. Latha

Effect of Water Probiotics on Growth Performance of Channa punctatus

R.Arthi Manju, J.Felicitta, M.Sakthivel, M.A.Haniffa, S.Valliammal and G.Chelladurai

Volume No. 1                                             November,2011

Table and Contents
Volume No. 1

An efficient protocol has been developed for clonal propagation from the of internodal regions of Tylophora indica (Burm F.) Merrill through successive in vitro trails. Basal medium of Murashige and Skoog, (1962) added with the combination of different levels of cytokinin and auxin molarity and concentrations were prepared. The effective suitable combinations were 3.25µM/l BA + 1.50 µM /l IAA, 12.25 µM/l KN +3.75 µM/l IBA and 1.25 µM/l Zeatin + 2.75 µM/l for calli, shoots elongation and roots formed in the inter nodal region of Tylophora indica respectively. The highest weight of calli formed in the optimum effective combination of 3.25µM/l BA + 1.50 µM /l IAA was observed in 8 week old cultures. The best effective combinations for shoot proliferation were 3.25µM/l BA + 1.50 µM /l IAA and 12.25 µM/l KN +3.75 µM/l IBA. The optimum rooting auxin concentration was 1.25 µM/l Zeatin + 2.75 µM/l for root formation. The present report serves as an effective protocol for the mass multiplication of Tylophora indica (Burm F.) Merrill in future. Tylophora indica /micropropagation

The antagonistic action of the marine sediment associated bacteria from three different area of Gulf of Mannar Coast was tested against five bacterial fish/shrimp pathogens viz. Aeromonas hydrophila, A.sobria, Vibrio vulnificus, Vibrio harveyi and V. fischeri using the double agar overlay method. The total bacterial load of sediment ranged from 105 -106 cfu/g. The bacterial composition of marine sediments predominantly consisted of Bacillus sp. (40%), followed by Vibrio sp., Flavobacterium sp., Alteromonas sp., Micrococcus sp., Staphylococcus sp. and Pseudomonas sp. Phylogenetic analysis of the sediments associated producer strains showed that 12 strains are clustered within the Firmicutes group belonging to several Bacillus sp. and Halobacillus sp. with 94 - 98% similarity between them. Of the 35 isolates tested, 42% showed antagonistic action against fish/shrimp pathogens. The marine sediment associated bacteria shall therefore be evolved as putative probiotic bacteria to counteract fish/shrimp disease problems. Marine biology/Molecular Biology

This study was investigated the nephroprotective activity of Solanum nigrum in Amphotericin B (AmB) induced nephrotoxicity in Wister Rats. The nephroprotective effect was analyzed by biochemical parameters such as haematological, biochemical parameters, antioxidants activity. The result shows that the hematological parameters such as RBC, WBC, Hb, PCV and platelet count were significantly decreased in AmB induced nephrotoxic rats. Biochemical parameters such as urea, BUN, uric acid and creatinine levels were significantly increased, total protein and albumin level were significantly reduced in AmB induced nephrotoxic rats. Enzymatic antioxidants and non-enzymatic antioxidants levels were significantly decreased in nephrotoxic rats. Pretreatment with aqueous extract of S. nigrum prevent the biochemical changes which near to normal suggest that S. nigrum has nephroprotective activity.

A study was carried out to investigate the effects of GA3, hot and cold water treatments on the germination of Strychnos potatorum L.f. The highest germination was recorded in seeds treated with 1000 ppm GA3 concentration with 24 hours soaking period. Germination was enhanced by increase in the GA3 concentrations in all the trials.

 Seed germination / Strychnos potatorum.

Effect of water probiotics for Channa punctatus based on growth performance, food conversion ratio and gut microbial load was investigated. C. punctatus (2.80-2.83g) was assigned to four treatments, with three replicates each. T1 (0.2mg of Lactic acid bacillus / 1000ml of water), T2 (0.4mg of Lactic acid bacillus / 1000ml of water), T3 (0.6mg of lactic acid bacillus /1000ml of water feed) and T4 (0.8mg of Lactic acid bacillus / 1000 ml of water). Fish were fed frequently with a diet of 40% crude protein at a rate of 4% of live body. After 45- days of feeding experiment with control diets, the treated with probiotic showed significantly better results of growth performances and FCR than those with the control diet. There was a difference between the treated fishes and control fishes. The obtained results showed that the weight gain, food conversion ratio, specific growth rate and survival rate were better in higher concentration of probiotics treated in water. The total heterotrophic count in the initial stage was 3.4 X 104 and the final stage was 8.8 x106 . In conclusion that the probiotic treatment in C. punctatus of 0.8mg/1000ml water was recommended to stimulate productive performances. Channa punctatus/ Probiotics