The entomopathogenic fungi are one of the insect pathogen with significant host range and host specificity. Growth and conidial survival of entomopathogenic fungi can be affected by environmental factors or by biopesticides and chemical products used to protect crop plants. In this study compatibility of Beauveria bassiana and Verticellium lecanii with neem based commercial formulation (Nimbecidine) and effect of this pesticide on growth, colony formation and biomass production were studied. The formulations of pesticides were tested in the concentration of 0.2%, 0.4% and 0.6% for Nimbecidine. From this study, interesting observation was noted. Nimbecidine is highly compatible with B. bassiana V. lecanii at these concentrations.
Invertase production from Aspergillus spp M1 isolated from honeycomb
Mahesh Patil, Ravikiran Bhamre and Ulhas Patil
Micropropagation of Trichosanthes anguina L. via cotyledonary node
A. Ambetkar, C. Uma Maheswari, Shantha Margaret, G. Sivanandhan, and N. Selvaraj
Compatibility of neem based oil nimbicidine with entomopathogenic fungi
N. Subbulakshmi, S.Ramarathinam, J.Anburaj, C.Sundaravadivelan, T.Kuberan, P.Kumar, T.Starlin and Marimuthu
Volume No. 4 February,2012
A newly isolated strain Aspergillus spp M1 was obtained from infected honeycomb. The strain produces efficient thermostable invertase which was active in acidic pH. The partially purified invertase was optimally active at pH 6 and 600C. The enzyme activity was enhanced by Ca2+ and Cd2+, while Hg2+ inactivates the enzyme. The maximum invertase production (238.18 U/ml) was detected by using sucrose (10 % w/v) as a carbon source while sodium nitrate (2.0 g/l) was found to be the best nitrogen source and supported maximum invertase production (233.27U/ml) in static condition.
Micropropagation of Trichosanthes anguina L. (Snake Gourd) was achieved using cotyledonary node explants derived from 7day old in vitro grown seedlings in a two step regeneration process. In the first step, the explants were cultured for four weeks for shoot bud induction and emergence of shoots in MS medium containing BA (0.2-1.4 mg/l) or Kn (0.2-1.2 mg/l) along with NAA (0.2 mg/l). At the end of the first step, higher number of shoots (14 shoots/explant) was produced in MS medium fortified with BA (1.2 mg/l), Kn (1.0 mg/l) along with NAA (0.2 mg/l). Individual effect of BA (1.2 mg/l) and Kn (1.0 mg/l) resulted in lower number of shoots induction with 12 and 7 shoots/explant respectively. In the second step, the explants with emerging shoots were subcultured in MS medium amended with BA (1.2 mg/l), Kn (1.0 mg/l) and L-glutamine (20 mg/l), which resulted in the maximum proliferation of shoots (18 shoots/explant). Elongation of shoots was obtained in MS medium containing GA3 (0.5 mg/l). Highest percentage (94.6) of root induction was achieved in MS medium supplemented with IBA (0.4 mg/l). Wellrooted plants were transferred to paper cups containing sand, soil and farmyard manure (1:1:1; w/v) and then to larger earthen pots. 90% of acclimatized plants survived in field.
