International Journal of  

                       Applied Bioresearch (IJAB)

ISSN 2250 - 2033 (Print); e -ISSN: 2250 - 2041 (Online)

Molecular Marker (Rapd) Based Fingerprinting on Jackfruit to Estimate the Genetic Diversity

Gopalsamy, J., Anburaj, J., Sundaravadivelan, C., Kuberan, T., Kumar, P., Starlin, T and M. Mariselvan

Volume No. 7                                                                                                       15 May, 2012

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Jackfruit (Artocarpus heterophyllus Lamk.) belongs to the family Moraceae. It grows abundantly in Bangladesh, India and in many parts of Southeast Asia. The fruit has been reported to contain high levels of protein, starch, calcium and thiamine. In Bangladesh, it is commonly referred to as "poor man's food". Many parts of the plant including the bark, roots, leaves and fruit, have medicinal properties. The leaf samples of the selected jackfruit varieties were collected, air dried and powdered using liquid nitrogen and was found suitable for further isolation. High quality of DNA getting in five varieties of jackfruit viz.TCJ4, TCJ2, FH10, FH4, and NC2. Amplification of jack fruit DNA for RAPD analysis using OPC7-GTCCGACGACGA was analyzed. The PCR reaction mixture was found optimum to produce intense and reproducible banding patterns in jack fruit. The dendrogram based on Euclidean distance was constructed using the computer package ‘STATISTICA’. This dendrogram indicated a moderate diversity among the five jack fruit varieties. Three major clusters were identified, one cluster with 2 varieties. The individuals in a cluster share common phylogenetic characteristics. This investigation to enables the identification and selection of jack fruit varieties for breeding programs and effective germplasm management.