International Journal of  

                       Applied Bioresearch (IJAB)

The quarterly peer-reviewed journal

ISSN 2250 - 2033 (Print); e -ISSN: 2250 - 2041 (Online)

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INDIA
GTRP- BIOJOURNALS

 

Isolation of cellulolytic fungi and their degradation on cellulosic agricultural wastes

 

Sivaramanan

 

Pigment producing coelomycetes endophytic fungus Bartalinia sp. isolated from Ixora coccinea L.

M. Murugan and S.Mugesh

Volume No. 19                                            December, 2013

Table and Contents

Abstract

 

Several tones of agricultural wastes are burnt and dumped annually worldwide. However, certainly there is more than one way to reuse them in productive manner, such as production of biogas and organic fertilizer. In the production of biogas fungal cellulolytic enzymes can be used as pretreatment agent for the fermentation process. Here 21 different fungi were cultured and among those higher cellulose degrading varieties were screened, extracted enzymes were examined for their activity by filter paper assay and DNS reagent method. In addition, further cultures are made on rice straw which is a common agro-waste in Asia. In straw substrate highest activity of 42.61 FPU/ml was denoted by Helminthosporium sp. where the saccharification was 3.8%, it is seconded by Cladosporium sp. where the activity was 40.36 FPU/ml. Enzymes of Trichoderma sp. and Aspergillus sp. also showed considerable activity.

Fungal metabolites are the identical contributor to the human welfare. Fungal pigment attained industrial demand for their timely requisite and biological importance to the environment. Endophytic fungus DMTMMM001 was identified as Bartalinia pondoensis by morphological and molecular identification using light microscope and analyzing 18s rRNA. Fungal pigments are reached for their adequate reproducibility and healthcare about the notorious effect of synthetic pigments. New source of the pigments has raising the industrial concern. In this study, we investigated pigmentation of fungal endophyte, which is isolated from medicinal plant Ixora coccinea. Keywords: Coelomycetes, endophytic fungi, Ixora coccinea, pigment.