International Journal of Biological Technology
ISSN: 0976-4313 Print
Translate this page in your preferred language:
Home | About the Journal |Instruction to Authors | Submit to Manuscript | Current Volume | Archives | Editorial Board | Contact Us
INDIA
GTRP- BIOJOURNALS
PEER - REVIEWED JOURNAL
International Journal of Biological Technology
.jpg)
Seasonal Variations of essential oils components analysis of Ocimum basilicum l. and its potential of antimicrobial activity in Tamilnadu
M. Maridass
FISSD’S- Research Institute of Conservation Ecology (Run by Foundation for
Innovative Science and Socio Economic Development (FISSD’S), Arunagiri
Complex, Palayamkottai Tirunelveli, Tamilnadu-627002,India.
Email: drmaridass@gmail.com; Mobile : +91-9487567793
FISSD’S- Research Institute of Conservation Ecology (Run by Foundation for
Innovative Science and Socio Economic Development (FISSD’S), Arunagiri
Complex, Palayamkottai Tirunelveli, Tamilnadu-627002,India.
Email: drmaridass@gmail.com; Mobile : +91-9487567793
Abstract
Aim: The study medicinal plants of Ocimum basilicum L. (Lamiaceae) commonly known as sweet basil, and it has been used as a traditional medicinal plant. The plant parts of O. basilicum L for the treatment of several diseases such as coughs, headaches and Ashma. The aim of the present study, traditional medicinal plants of Ocimum basilicum L. grown in Tirunelveli, Tenkasi and Kanyakumari District of Tamilnadu was investigated for essential oil content and composition and its potential of antimicrobial activity. Methods: The fresh samples of Ocimum basilicum L. leaves were thoroughly washed in tap water and essential oils of were obtained by hydro distillation method. The content of essential oil of O. basilicum L. (v/w (% ) were collected in three regions of Tirunelveli, Tenkasi and Kanyakumari districts and its essential oils of O. basilicum L. were evaluation of antimicrobial potential observed by agar disk diffusion methods. The oils of Ocimum basilicum L. were analyzed and identified by GC and GC-MS methods. Results: The results of essential oils content of O.basilicum L. were observed maximum content of oils in Tenkasi followed by Kanyakumari and Tirunelveli. Major components of the essential oils were thymol (19.4-60.1%), γ-terpinene (0.3-13.8%) and p-cymene (3.5-10.4%). The results clearly indicated that season has significant effect on quality and quantity of thyme oil. The essential oils derived from O. basilicum demonstrated significant activity against Staphylococcus aureus and Aspergillus niger. The highest levels of activity for these essential oils were noted against all tested bacteria and fungi during the summer season.
Keywords: Ocimum basilicum L.; Essential oils; GC-MS methods; Antimicrobial activity; India.
Aim: The study medicinal plants of Ocimum basilicum L. (Lamiaceae) commonly known as sweet basil, and it has been used as a traditional medicinal plant. The plant parts of O. basilicum L for the treatment of several diseases such as coughs, headaches and Ashma. The aim of the present study, traditional medicinal plants of Ocimum basilicum L. grown in Tirunelveli, Tenkasi and Kanyakumari District of Tamilnadu was investigated for essential oil content and composition and its potential of antimicrobial activity. Methods: The fresh samples of Ocimum basilicum L. leaves were thoroughly washed in tap water and essential oils of were obtained by hydro distillation method. The content of essential oil of O. basilicum L. (v/w (% ) were collected in three regions of Tirunelveli, Tenkasi and Kanyakumari districts and its essential oils of O. basilicum L. were evaluation of antimicrobial potential observed by agar disk diffusion methods. The oils of Ocimum basilicum L. were analyzed and identified by GC and GC-MS methods. Results: The results of essential oils content of O.basilicum L. were observed maximum content of oils in Tenkasi followed by Kanyakumari and Tirunelveli. Major components of the essential oils were thymol (19.4-60.1%), γ-terpinene (0.3-13.8%) and p-cymene (3.5-10.4%). The results clearly indicated that season has significant effect on quality and quantity of thyme oil. The essential oils derived from O. basilicum demonstrated significant activity against Staphylococcus aureus and Aspergillus niger. The highest levels of activity for these essential oils were noted against all tested bacteria and fungi during the summer season.
Keywords: Ocimum basilicum L.; Essential oils; GC-MS methods; Antimicrobial activity; India.
