International Journal of  Biological Technology
ISSN: 0976-4313 Print
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INDIA
GTRP- BIOJOURNALS
PEER - REVIEWED JOURNAL
Archieves
Archieves

Volume   No.10(1)                             April,2019

Table and Contents

Effect of heat stress on free amino acids in leaves of Aloe vera and Bryophyllum pinnatum
 
BABITA  Kumari  and PRASHANT Kumar Roy
 
Faculty of Sciences, Indira Gandhi Technological and Medical Sciences University, Ziro, Arunachal Pradesh (India)
Department of Paramedical, Indira Gandhi Technological and Medical Sciences University, Ziro, Arunachal Pradesh (India)

Email: bbtmshr@yahoo.co.in
 

 
 

Abstract

In the present study the effects of heat stress on the free amino acid profiles of Aloe vera and Bryophyllum pinnatum leaves were investigated. Heat stress was provided by exposing the plants to a daily temperature of 38-43 0C for one month. After heat stress the leaves were collected and dried in oven. The powdered dried leaves were used for amino acids extraction and analysis. In Aloe vera leaves 9 essential and 7 non-essential amino acids were detected, highest concentration was recorded for phenylalanine followed by arginine and valine while histidine and aspartate were present in the lowest amounts.  In Bryophyllum pinnatum 8 essential and 7 non-essential amino acids were detected, glycine was recorded in highest concentrations followed by arginine and proline while lysine was observed in the lowest concentrations. The two plants accumulated different amino acids in response to heat stress. In both plant species proline and serine were accumulated while the concentration of alanine and glycine declined significantly in response to heat stress. The total essential amino acids decreased while total non-essential amino acid increased significantly in Aloe vera while in Bryophyllum pinnatum no significant effect was observed on the concentration of total essential and total non-essential amino acids in response to heat stress.
 
Keywords: Plants leaves, Heat stress, Free amino acids
Cytotoxic studies on medicinal of plants of Calatrophis procera and Euphorbia hirta latex
Muthiah MARIDASS and Ganapathy RAJU
 
Department of Zoology, Pioneer Kumaraswamy College, (Affiliated to Manonmaniam Sundaranar University), Nagercoil, Tamil nadu-629003, India.
Corresponding Author Email maridassugcpdf@yahoo.co.in
 
 

Brine shrimp lethality bioassay (BSLB) is considered as a useful tool for preliminary assessment of cytotoxicity studies. The present study was aimed to assess the cytotoxicity properties of both plants of Calatrophis procera and Euporbia hirta latex. The cytotoxic activity of both plants of C. procera and E. hirta latex was tested using the test animals of Artemia salina (Brine shrimp) bioassay. The percentage of mortality of both extracts was observed by LC50 values by the Finney probit analysis method. The results of both these plants have good cytotoxicity activities.
 
Keywords: Brine shrimp test, Cytotoxicity, Medicinal plants, Calotraphis procera, Euphorbia hirta

Volume No.10(1)