GC-MS Analysis of chemical Constituents and antibacterial activity of Indigofera aspalathoids D.C stem.

G. Raju, N. Subash and M. Maridass

Abtract

The whole plant of Indigofera aspalathoides DC has been medicinally used for cooling, demulcent and tumors, leprosy, cancer and skin disorders. The aim of present study was to analyse and identified in chemical composition of I. aspalathoides DC by Gas Chromatography–Mass Spectrometry (GC-MS) method and antibacterial activity of the hexane, chloroform and ethanol extract of I. aspalathoides DC. The results of I. aspalathoides stems were 21 chemical components identified by GC-MS method. The major compounds of terpenoids constituents were (all-Z)- Methyl eicosa-11,14-dienoate(16.84%), caparapidiol (11.11%), incensole (11.09%). The antibacterial activities of hexane and ethanol extract of I. aspalathoides were remarkable activity against Vibrio harvae (S.No.7771), Aeromonas hydrophila Sub sp. Hydrophila (S.No.1739) and A. sobria (S.No.1944). Chloroform extract of I. aspalathoides was moderate active against Aeromonas hydrophila Sub sp. Hydrophila (S.No.1739) and Aeromonas sobria (S.No.1944) and no activiy against Vibrio harvae. The conclusion of the present study indicates that phytochemical constituents of I. aspalathoides DC stem have a potential for antibacterial compounds for fish forming.

Keywords: Indigofera aspalathoides DC, terpenoids, Chromatography–Mass Spectrometry (GC-MS) method, antibacterial activity

Evaluation of antimicrobial compounds synthesized by endophytic fungi isolated from five Indian medicinal plants

Prabha Palanichamy, Govindan Krishnamoorthy, K.Thangavel and Murugan Maruthamuthu

Abstract: Antibiotic resistance in microbes has grown into a global concern, hence the exploration of new antibacterial agents from natural sources is an emerging area of research. Endophytic fungi were isolated from five medicinal plants in southern Tamil Nadu and were explored for their antimicrobial potential. Endophytic fungui isolated from the leaves of the medicinal plants were grown in potato dextrose broth for 21 days. The culture filtrate was extracted with ethyl acetate. The antimicrobial activity was evaluated against bacterial pathogens such as Escherichia coli, Corynebacterium diphtheriae, Methicillin Resistant Staphylococcus aureus (MRSA), Proteus mirablis and Salmonella typhi. The extract from endophytic fungi VN-1 and OB-1 exhibited an effective antibacterial activity against all the pathogens. JJ-5 fungal extract showed moderate activity against the pathogens. The extracts from JJ-1, JJ-4, CS-3 and GP-1 exhibited very less activity against the pathogens. This study shows that the extracts from endophytic fungi isolated from medicinal plants will serve as a potential source for the production of effective antibacterial compounds. Keywords: Medicinal plants, Endophytic fungi, Antibacterial assay, Ethyl acetate.

Volume No. 3(2)           December,2013

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